CFX96TOUCH Fluorescent Quantitative PCR sesebelisoa

CFX96Touch fluorescent quantitative PCR e ka sebelisoa mafapheng a fapaneng a lipatlisiso tsa nucleic acid quantification, tlhahlobo ea boemo ba liphatsa tsa lefutso, ho lemoha phetoho ea liphatsa tsa lefutso, ho lemoha GMO, le tlhahlobo e khethehileng ea sehlahisoa.

Tikoloho ea Mosebetsi:
1.1 Mocheso o sebetsang: 5-31 ° C
1.2 Ho sebetsa le mongobo: mongobo o lekanyelitsoeng ≤80%
1.3 Matla a sebetsang: 100-240 VAC, 50-60Hz
Litlhoko tsa tšebetso le tekheniki tsa CFX96Touch fluorescence quantitative PCR
3.1 Ts'ebetso ea mantlha (* ke letšoao le lokelang ho finyelloa)
* 3.1.1 Mekhoa e tšeletseng ea teko, 5% PCR e ka fumanoa, 'me liphatsa tsa lefutso tsa 5 li ka fumanoa ka nako e le' ngoe, 'me mocha o khethehileng oa ho lemoha FRET o fumanoa ka nako e le' ngoe.
* 3.1.2 Ka ts'ebetso e matla ea mocheso oa PCR, o ka tsamaisa lithempereichara tse 8 tse fapaneng ka nako e le 'ngoe, ho kenya mocheso o mong le o mong.
3.1.3 Ho buloa ha li-reagents tse felletseng, lipatlisiso tse fapaneng le lisebelisoa tsa bongaka lia sebetsa;
3.1.4 E loketse mefuta e fapaneng ea mekhoa ea fluorescence joalo ka Taqman, Molecular Beacon, Fret probe, Sybr Green i, joalo-joalo;
3.1.5 Open, 0.2ml single tube, octal, 96-well plate, joalo-joalo.
* 3.1.6 e ka sebetsa ka boikemelo, ts'ebetso ea 'nete e kantle ho marang-rang, ha ho na tlhoko ea ho hokela komporo ho beha leihlo curve ea amplification ea PCR fluorescent ka nako ea nnete;
3.2 Litlhoko tsa mantlha tsa tekheniki (* ke letšoao le lokelang ho finyelloa)
* 3.2.1 Bokhoni ba sampuli: 96 × 0.2ml, litlhaloso tse tloaelehileng tsa lipoleiti tse 96 (12 × 8) li ka sebelisoa;
Mofuta oa 3.2.2 oa Lisebelisoa: 0.2ml tube e le 'ngoe, ho kopanya tse robeli, lipoleiti tsa 96-liba, joalo-joalo.
3.2.3 Mokhoa oa ho itšoara: 1-50μL (e khothalletsoa 10-25 μL);
* 3.2.4 Mohloli oa leseli: Li-LED tse tšeletseng tse nang le li-filters;
* 3.2.5 Detector: Li-diode tse tšeletseng tsa lifoto tse nang le li-filters;
* 3.2.6 litha e le nngwe lebelo la ho pholile: 5 ° C / sec;
3.2.7 Lebelo la ho laola mocheso: 0 -100 ° C;
3.2.8 Mocheso o Nepahetseng: ± 0.2 ° C (90 ˚C);
3.2.9 Ho lumellana ha mocheso: ± 0.4 ° C (90 ˚C nakong ea metsotsoana e 10);
* 3.2.10 Mosebetsi oa Mocheso o Matla oa Gradient: Matha lithempereichara tse 8 tse fapaneng ka nako e le 'ngoe; mefuta ea taolo ea mocheso oa gradient: 30 -100 ° C; mocheso o fapaneng oa mocheso o fapaneng: 1 - 24 ° C; gradient mocheso incubation nako: e tšoanang;
3.2.11 excitation / emission wavelength range: 450-730 nm;
3.2.12 Boikutlo: Kopi e le 'ngoe ea lefutso ho genome ea motho e ka bonoa;
3.2.13 Dynamic range: 10 palo;
3.2.14 Pontšo: 8.5 inch color touch screen;
3.2.15 Mokhoa oa ho hlahloba lintlha: Standard Curve Quantity, Melting Curve, CT kapa ΔΔCT Gene Expression Analysis, Multiple Internal Genodes Analysis le Amplification Efficiency Calculation, Multiple Data Files Gene Expression Analysis, Allelic Analysis, Type Analysis, Have Gene, melting curve analysis function. ;
3.2.16 Thomello ya Boitsebiso: Excel, Word, kapa PowerPoint. Tlaleho ea mosebelisi e na le litlhophiso tse tsamaisang, lits'oants'o, le liphetho tsa data tsa litafole, tse ka hatisoang kapa tsa bolokoa joalo ka PDF;
* 3.2.17 Lithuto tsa sebopeho sa Chromosomal: Mokhoa oa tlhahlobo ea bongata ba libopeho tsa chromatin ka ho bapisa karolo ea ho senyeha ha DNA ea genomic ka karolo e bapisang ea DNA ea genomic. E hlile e paka kamano ea bophahamo pakeng tsa sebopeho sa chromatin le polelo ea liphatsa tsa lefutso;