Ho ea ka molao-motheo oa qeto ea naetrojene ea Kjeldahl, ho hlokahala mehato e meraro bakeng sa boikemisetso, e leng tshilo ya dijo, distillation le titration.
Tšhilo ea lijo: Chesa metsoako ea nitrogen e nang le nitrogen (liprotheine) hammoho le concentrated sulfuric acid le catalysts (copper sulfate kapa Kjeldahl digestion tablets) ho senya protheine. The carbon le hydrogen li oxidized ka carbon dioxide e le metsi ho baleha, ha organic Nitrogen e fetoloa ammonia (NH3) le ho kopanngoa le sulfuric acid ho etsa ammonium sulfate. (Ammonium NH4+)
Ts'ebetso ea tšilo ea lijo: ho futhumatsa ka mocheso o tlase ho pheha, ntho e ka har'a botlolo e entsoe ka carbonized mme e ntšo, 'me ho hlahisoa foam e ngata. Ka mor'a hore foam e nyamele, eketsa matla a mollo ho boloka boemo bo fokolang ba ho bela. Ha mokelikeli o fetoha o moputsoa-botala le o hlakileng, tsoela pele ho futhumatsa bakeng sa 05-1h, 'me u pholile ka mor'a bofelo. (U ka sebelisa sesebelisoa sa tšilo ea lijo ka boiketsetso ho phethela mosebetsi oa pele ho ts'ebetso)
Distillation: Tharollo e fumanoeng e hlapolloa ka molumo o sa khaotseng ebe e kenngoa ka NaOH ho lokolla NH3 ka distillation. Ka mor'a condensation, e bokelloa ka tharollo ea boric acid.
Ts'ebetso ea distillation: Ntlha ea pele, sampuli e hloekisitsoeng e hlapolotsoe, NaOH e eketsoa, 'me khase ea ammonia e hlahisoang ka mor'a hore ho futhumale e kene ka har'a condenser, ebe e phalla ka har'a botlolo e amohelang e nang le tharollo ea boric acid ka mor'a hore e fokotsehe. E etsa ammonium borate. (Letšoao le tsoakiloeng le kenyelletsoa tharollong ea boric acid. Ka mor'a hore ammonium borate e thehoe, tharollo e monyang e fetoha ho tloha ho asiti ho ea ho alkaline, 'me 'mala o fetoha ho tloha ho pherese ho ea ho o moputsoa-botala.)
Titration: Titrate e nang le hydrochloric acid standard solution ea concentration e tsebahalang, bala litaba tsa naetrojene ho latela bongata ba hydrochloric acid e sebelisitsoeng, ebe u e atisa ka ntlha e tsamaisanang le phetoho ho fumana protheine. (Titration e bolela mokhoa oa tlhahlobo ea bongata le ts'ebetso ea liteko tsa lik'hemik'hale. E sebelisa mokhoa oa ho etsa lipalo oa litharollo tse peli ho fumana hore na solute e itseng ke efe. E bontša ntlha ea ho qetela ea titration ho latela phetoho ea 'mala oa letšoao, ebe o bona ka mahlo tšebeliso ea tharollo e tloaelehileng ea Volume, lipalo le liphetho tsa tlhahlobo.)
Titration process: Lahlela tharollo e tloaelehileng ea hydrochloric acid ka har'a tharollo ea ammonium borate ho fetola 'mala oa tharollo ho tloha ho botala bo boputsoa ho isa bofubelu bo khanyang.
DRK-K616 automatic Kjeldahl nitrogen analyzer ke analyzer e bohlale ea othomathike bakeng sa ho fumana litaba tsa naetrojene ho latela mokhoa oa Kjeldahl. E ka sebelisoa haholo ts'ebetsong ea lijo, tlhahiso ea lijo, koae, leruo la liphoofolo, manyolo a mobu, tlhokomelo ea tikoloho, meriana, temo, lipatlisiso tsa saense, thuto, tlhokomelo ea boleng le likarolo tse ling bakeng sa tlhahlobo ea naetrojene le protheine ho macro le semi-micro. mehlala. E ka boela ea sebelisoa bakeng sa letsoai la ammonium, Ho lemoha ha mafura a mafura a mafura / alkali, joalo-joalo Ha u sebelisa mokhoa oa Kjeldahl ho fumana sampuli, e hloka ho feta ka mekhoa e meraro ea tšilo ea lijo, distillation le titration. Distillation le titration ke mekhoa e meholo ea ho lekanya ea DRK-K616 Kjeldahl nitrogen analyzer. Mofuta oa DRK-K616 oa mofuta oa Kjeldahl nitrogen analyzer ke mokhoa oa ho lekanya naetrojene o itirisang ka botlalo le mokhoa oa ho lekanya naetrojene o etselitsoeng ho latela mokhoa oa khale oa ho fumana naetrojene ea Kjeldahl; sesebelisoa sena se fana ka boiketlo bo boholo bakeng sa bahlahlobi ba laboratori nakong ea ho khetholla naetrojene-protheine. , E na le litšobotsi tsa tšebeliso e sireletsehileng le e ka tšeptjoang; tshebetso e bonolo le ho boloka nako. Sebopeho sa Sechaena sa lipuisano se etsa hore mosebedisi a be bonolo ho sebetsa, sebopeho se na le botsoalle, 'me boitsebiso bo bontšitsoeng bo ruile, e le hore mosebedisi a ka utloisisa ka potlako tšebeliso ea sesebelisoa.
Nako ea poso: Feb-25-2022
